AI 菌落計數器使用指南 (AI Colony Counter User Guide)

上傳培養皿照片後，AI 會自動偵測並標記菌落，使用者可框選計數範圍、手動新增／移除標記進行校正，並自動計算轉形效率（TE），支援連續拍攝多個培養皿並彙整成一份結果紀錄表。

Upload a photo of your agar plate and the AI will automatically detect and mark colonies. You can select the counting region, manually add or remove markers to correct the result, and the tool will automatically calculate transformation efficiency (TE) — with support for logging multiple plates into a single results table.

Step 1　上傳培養皿照片 (Upload Plate Photo)

• 中文說明：點擊「現場拍照」以相機即時拍攝，或點擊「從相簿選擇」上傳現有照片。拍攝建議：關閉閃光燈、正上方垂直拍攝、避免燈光直接反光在培養皿上，並盡量讓培養皿佔滿畫面，以提升 AI 辨識準確度。

• English Instructions: Tap "Take Photo" to capture a plate image with your camera, or "Choose Gallery" to upload an existing photo. Tips: turn off the flash, shoot straight down from above, avoid direct glare on the lid, and fill the frame with the plate for best AI accuracy.

Step 2　TE 批次參數設定（選填）(TE Batch Settings — Optional)

• 中文說明：若要連續拍攝多個培養皿，可預先輸入固定不變的「投入 DNA 質量」「回收體積」「塗盤體積」。只要不手動修改，之後每拍一張新照片都會自動沿用同一組數值，方便快速比較不同培養皿的轉形效率。

• English Instructions: If you plan to photograph several plates in a row, you can pre-set the fixed "Input DNA Mass," "Recovery Volume," and "Plated Volume" here. As long as these values aren't changed manually, every new photo will reuse the same settings — useful for quickly comparing TE across plates.

Step 3　框選計數範圍 (Select Region of Interest)

• 中文說明：選擇圓形、矩形或自由框選（至少 3 點）三種形狀，拖曳中心或邊緣控制點調整範圍，盡量貼合培養皿內緣、避開反光或邊緣模糊區域；框選範圍外的 AI 偵測結果將自動被過濾。完成後按「確認範圍，開始 AI 分析」。

• English Instructions: Choose a Circle, Rectangle, or Freeform (minimum 3 points) shape. Drag the center or edge handles to fit the region tightly to the plate, avoiding glare or blurred edges — detections outside the selected region are automatically filtered out. Then tap "Confirm ROI & Run AI" to start analysis.

Step 4　AI 辨識結果與手動校正 (AI Detection & Manual Correction)

• 中文說明：AI 分析完成後，畫面會顯示自動標記的菌落數。切換「新增」模式並點擊照片可補上漏判的菌落；切換「移除」模式並點擊標記可刪除誤判項目；數目會即時更新。畫面可縮放（滾輪／按鈕/雙擊）並拖曳平移，方便處理密集重疊的菌落；若操作有誤，可按「復原上一步」（或 Ctrl+Z）逐步撤銷。

• English Instructions: Once AI analysis completes, the detected colony count is displayed on the photo. Switch to "Add" mode and tap the image to mark missed colonies; switch to "Remove" mode and tap a marker to delete a false detection — the count updates in real time. Zoom (scroll, buttons, or double-tap) and pan to handle densely overlapping colonies; use "Undo" (or Ctrl+Z) to step back through changes if needed.

Step 5　標記樣式設定 (Marker Style Settings)

• 中文說明：可自訂 AI 偵測與手動新增標記的樣式（點點／圈選／十字）、顏色（預設色票或自訂色）以及點點大小，方便在不同背景的培養皿照片上清楚辨識標記。

• English Instructions: Customize the marker shape (Dot / Circle / Cross), color (preset swatches or a custom picker) for AI-detected versus manually added points, and the dot size — making markers easy to distinguish against plates of different backgrounds.

Step 6　轉形效率（TE）計算 (Transformation Efficiency Calculator)

• 中文說明：菌落總數會自動同步偵測結果（也可按「同步偵測數目」手動更新），系統依公式「TE = 菌落數 ÷ [DNA 質量(µg) × (塗盤體積 ÷ 回收體積)]」計算 TE，並依數值高低顯示效率評級（如優異／普通／偏低）與說明建議。可選填「盤子名稱／實驗條件」以利後續辨識。

• English Instructions: The total colony count automatically syncs with the detection result (or tap "Sync Count" to update manually). TE is calculated using the formula "TE = Colonies ÷ (DNA mass in µg × plated vol / recovery vol)," and the result is shown with an efficiency rating (e.g. Excellent / Fair / Low) and explanatory notes. You may optionally fill in a "Plate Name / Condition" for easier identification.

• Step 7　記錄與多盤結果彙整 (Log Results & Multi-Plate Table)

• 中文說明：按「記錄此盤，拍下一盤」會將目前的名稱、CFU、TE 及當下使用的 DNA／體積參數存入下方紀錄表，並自動開啟相機拍攝下一張；若已是最後一盤，改按「記錄此盤（最後一盤）」即可停留在目前結果並捲動至紀錄表確認。紀錄表可編輯盤子名稱、單筆刪除，或按「清空紀錄」全部清除。

• English Instructions: Tap "Log & Take Next Photo" to save the current name, CFU, TE, and the DNA/volume settings in use at that time into the log table below, then automatically open the camera for the next plate. If this is the last plate, use "Log (Last Plate)" instead to stay on the current result and scroll to the log table. Entries in the log can be renamed, deleted individually, or cleared entirely via "Clear Log."

降低誤判的建議 (Tips to Reduce Miscounts)

• 中文：若邊緣菌落被切到或框到反光區域，請按「重新框選」調整範圍後重跑 AI；若菌落密集重疊，建議放大畫面用「新增」補上遺漏菌落；若因反光或閃光造成誤判，可用「移除」清除，或重拍時關閉閃光燈。

• English: If an edge colony is cut off or glare falls inside the selection, redraw the ROI and rerun the AI. For densely overlapping colonies, zoom in and use "Add" to mark missed ones. For false detections caused by glare or flash, use "Remove," or reshoot with the flash off.